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KMID : 0380119780020000015
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Korean Journal of Ecology
1978 Volume.2 No. 0 p.15 ~ p.22
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Studies on the crown gall disease of chestnut nurse seedling grafts
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羅瑢俊/La, Yong Joon
任炯彬/李光然/李斗珩/Im, Hyong Bin/Lee, Kwang Yeun/Lee, Du Hyung
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Abstract
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Pathological and anatomical studies on the cause of the crown gall like symptoms associated with the chestnut nurse grafts were undertaken. The crown gall bacterium, Agrobacterium tumefaciens, was isolated from the gall tissues of chestnut nurse grafts by using selective media developed by Schroth et al. and Kado and Heskett.
Typical crown gall symptoms appeared on tomato, castor bean and geranium plants 10∼21 days following inoculation with the bacterium isolated from the gall tissues of chestnut nurse grafts. Agrobacterium tumefaciens was reisolated from crown gall tissues of tomato, castor bean and geranium. Anatemical studies on the origin, growth and differentiation of the gall tissues of the chestnut nurse grafts confirmed that the gall tissues are of crown gall origin.
Masses of Agrobacterium tumefaciens were observed from gall tissues of chestnut nurse grafts, so it could be confirmed that the crown gall symptoms prevalent on chestnut nurse grafts are caused by the crown gall bacterium, Agrobacterium tumefaciens.
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